Plasmid_Backbone

Part:BBa_K4782010:Design

Designed by: Adina Kadyrova   Group: iGEM23_NU-Kazakhstan   (2023-10-12)


pET 9a low copy plasmid


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 588
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal NheI site found at 229
    Illegal NheI site found at 543
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BglII site found at 646
    Illegal BamHI site found at 510
    Illegal XhoI site found at 3552
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 588
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 588
    Illegal NgoMIV site found at 678
    Illegal NgoMIV site found at 1046
    Illegal NgoMIV site found at 1206
    Illegal NgoMIV site found at 1560
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 607
    Illegal SapI site found at 2629


Design Notes

addgene database

Source

addgene database

References